We confirmed the activation of Akt signaling by western blot using an Akt signaling array following the manufacturer's instruction (www.raybiotech.com). This kit was a slide-based antibody array founded upon the sandwich immunoassay principle. Each dot represented phosphorylation protein within the Akt signaling pathway that was listed in Fig. 3b. Target-specific capture antibodies, biotinylated protein (positive control), and nonspecific IgG (negative control) were included in duplicate onto the glass slides.
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